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Gas Chromatography Although capillary tubing is now used over a broad range of applications, its history is inseparable from that of Gas Chromatography. According to the International Union of Pure and Applied Chemistry (IUPAC), “Chromatography is a physical method of separation in which the components to be separated are distributed between two phases, one of which is stationary (stationary phase) while the other (the mobile phase) moves in a definite direction”.2 In Gas Chromatography (GC), the mobile phase is a gas and the stationary phase is either a solid or a liquid, depending on the type of separation column selected. The actual separation of components in a mixture is achieved due to the differential distribution of solutes between the mobile and stationary phases. Capillary is fundamental to the technique, as it is the support for the stationary phase and the conduit through which the mobile phase travels. One of the first applications for capillary tubing was in the Gas Chromatography industry. Dandeneau and Zerenner’s pioneering efforts are recognized by many as the cornerstone for the introduction of fused silica based capillary as a support for GC columns.3 Continued improvements in GC analysis have been aided by the unique properties of synthetic fused capillary tubing, with the majority of new methods rooted in proprietary new stationary phase coatings. Since its first use in the late 70’s, the range of capillary tubing internal diameters (ID) has grown, and specification tolerances have continually tightened to meet the exacting demands of the column manufacturing industry. Numerous GC column manufacturers offer a broad selection of columns used in the analysis of food products, pharmaceuticals, clinical samples, petroleum, and a variety of other applications. Polymicro produces a wide range of capillary designed specifically for GC applications. Capillary with ID’s ranging from 50mm up to 750mm are routinely used, with the majority of columns employing capillary between 180mm and 530mm. Two product lines are commonly used, these being the standard TSP and high temperature TSG. Although Polymicro communicates the specifications in micrometers, most column manufacturers list column diameters in millimeters. It is common to name a column by a combination of the ID and stationary phase. 2 McNair, H. M., Miller, J. M., Basic Gas
Chromatography, Chapter 1, John Wiley & Sons, Inc. New York, (1997)
The information given herein, including drawings, illustrations and schematics (that are intended for illustration purposes only), is believed to be reliable. However, Polymicro Technologies makes no warranties as to its accuracy or completeness and disclaims any liability in connection with its use. Polymicro Technologies only obligation shall be as set forth in Polymicro Technologies' standard terms and conditions of sale for this product and in no way will Polymicro Technologies be liable of any incidental, indirect or consequential damages arising out of the sale, resale, use or misuse of the product. Users of Polymicro Technologies' products should make their own evaluation to determine the suitability of each such product for the specific application.
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Copyright © Polymicro Technologies, 1995 - 2008 All Rights Reserved
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